Extraction and comparison of quality and purity of DNA of medicinal plants by CTAB and kit method

Raheela Jabeen; Ume Habiba; Sana Khalid; Jafir Hussain Shirazi

doi:10.56770/jcp2022613

Authors

Raheela Jabeen Department of Biochemistry and Biotechnology, The Women University Multan
Ume Habiba University College of Conventional Medicine, Faculty of Allied Health Sciences, The Islamia University of Bahawalpur, Pakistan
Sana Khalid Department of Microbiology and Molecular Genetics, The Women University Multan, Pakistan
Jafir Hussain Shirazi Department of Pharmaceutics, Faculty of Pharmacy, The Islamia University of Bahawalpur

DOI:

https://doi.org/10.56770/jcp2022613

Keywords:

CTAB, DNA, Cassia fistula, Albizzia lebbeck, Saccharum officinarum, Cymbopogan citrates

Abstract

Background: Today is a world of bioengineering. Man is playing with DNA. Extraction and isolation of DNA has become as a key part for the researches of modern times. One needs high quality and increased quantity of DNA for performing several genetic techniques. There are several methods through which DNA can be extracted but the quality of the DNA cannot be compromised. Material methods: In the current research work extraction of DNA from four medicinal important plants Cassia fistula, Saccharum officinarum, Albizzia lebbeck and Cymbopogan citratus was carried out by two methods. One is a manual method that is used for the extraction of DNA from plant source; The CTAB method, and the other is extraction with the help of commercially available kit. Results: Results of the work showed that the high quantity of the DNA was obtained by CTAB method while DNA extracted with the kit was of better quality and purity then CTAB method. Conclusion: In techniques where high quality DNA is required like genetic engineering, micro arrays, extraction of DNA through kit method should be preferred, while in cases where quantity of DNA maters over its quality CTAB method is better to be used and economic.